Difference between revisions of "Part:BBa J70620:Design"
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===Design Notes=== | ===Design Notes=== | ||
Primers: | Primers: | ||
| − | + | GTTTCTT C GAATTC GCGGCCGC T ACTAGT AAAATCGAAGAAGGTAAACTGGTAATCTGG, malE-F | |
| − | + | GTTTCTT C CTGCAG CGGCCGC T GCTAGC GTTGTTGTTATTGTTATTGTTGTTGTTGTTCGAG, malE-RN | |
| + | GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGTCTGCGCGTCTTTCAGGGC, malE-R | ||
===Source=== | ===Source=== | ||
Latest revision as of 15:09, 9 August 2010
RFC12 Maltose Binding Protein Internal Domain [malE]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 357
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 55
Design Notes
Primers:
GTTTCTT C GAATTC GCGGCCGC T ACTAGT AAAATCGAAGAAGGTAAACTGGTAATCTGG, malE-F GTTTCTT C CTGCAG CGGCCGC T GCTAGC GTTGTTGTTATTGTTATTGTTGTTGTTGTTCGAG, malE-RN GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGTCTGCGCGTCTTTCAGGGC, malE-R
Source
NEB:[http://www.neb.com/nebecomm/products/productn8076.asp pMAL-c2x]
References
<biblio>
- Kimple pmid=18429272
- Terpe pmid=12536251
</biblio>