Difference between revisions of "Part:BBa B0012:Design"
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<partinfo>BBa_B0012 short</partinfo> | <partinfo>BBa_B0012 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). | Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). | ||
Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. | Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. | ||
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Revision as of 03:12, 6 May 2006
TE from coliphageT7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).
Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.