Difference between revisions of "Part:BBa K390006"

Latest revision as of 15:35, 2 August 2010

sigA Type I promoter from Synechocystis sp. PCC 6803

This is the promoter for the sigma factor sigA in Synechocystis sp. PCC 6803. It is self-regulating (controlled by SigA), but has shown to potentially be down-regulated due to changes from heat-shock, darkness, and salt stress. A decrease in SigA protein was observed in a SigC knockout, but not in direct proportion to the amount of SigA transcript (Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3:65-87).

As SigA is the housekeeping sigma factor for Synechocystis sp. PCC 6803, this promoter is likely constitutive, with a moderate level of expression (this is pending experimental verification).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K390006 short</partinfo>
-This is the promoter for the sigma factor ''sig''A in ''Synechocystis'' sp. PCC 6803. It is self-regulating (controlled by SigA), but has shown to potentially be down-regulated due to changes from heat-shock, darkness, and salt stress. A decrease in SigA protein was observed in a SigC knockout, but not direct proportion to the transcript of SigA (Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3:65-87).
+This is the promoter for the sigma factor ''sig''A in ''Synechocystis'' sp. PCC 6803. It is self-regulating (controlled by SigA), but has shown to potentially be down-regulated due to changes from heat-shock, darkness, and salt stress. A decrease in SigA protein was observed in a SigC knockout, but not in direct proportion to the amount of SigA transcript (Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3:65-87).
 As SigA is the housekeeping sigma factor for ''Synechocystis'' sp. PCC 6803, this promoter is likely constitutive, with a moderate level of expression (this is pending experimental verification).