Difference between revisions of "Part:BBa J72037:Design"
Jennbrophy (Talk | contribs) (→Source) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J72037 short</partinfo> | <partinfo>BBa_J72037 short</partinfo> | ||
| Line 14: | Line 13: | ||
PCR | PCR | ||
| − | + | Construction of MED7 Basic Part Bmll5 | |
| − | Construction of MED7 Basic Part Bmll5 | + | PCR MLL3/MLL78 on pBca9523-Bmll3 (324bp, EcoRI/BamHI) |
| − | + | Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L) | |
| − | PCR MLL3/MLL78 on pBca9523-Bmll3 (324bp, EcoRI/BamHI) | + | Product is pBca9523-Bmll5 |
| − | + | ------------------------------------- | |
| − | Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L) | + | MLL3 Forward Biobricking of Bmll5 CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA |
| − | + | MLL78 Reverse Biobricking of Bmll5 CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG | |
| − | Product is pBca9523-Bmll5 | + | |
| − | + | ||
| − | ------------------------------------- | + | |
| − | + | ||
| − | MLL3 Forward Biobricking of Bmll5 CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA | + | |
| − | + | ||
| − | MLL78 Reverse Biobricking of Bmll5 CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG | + | |
===References=== | ===References=== | ||
Latest revision as of 23:29, 15 July 2010
{Bmll5} in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
PCR
Construction of MED7 Basic Part Bmll5 PCR MLL3/MLL78 on pBca9523-Bmll3 (324bp, EcoRI/BamHI) Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L) Product is pBca9523-Bmll5 ------------------------------------- MLL3 Forward Biobricking of Bmll5 CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA MLL78 Reverse Biobricking of Bmll5 CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG