Difference between revisions of "Part:BBa J72031:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J72031 short</partinfo>
 
<partinfo>BBa_J72031 short</partinfo>
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Overlap Extension PCR (Wobble Construction)
 
Overlap Extension PCR (Wobble Construction)
 +
===Wobble construction of Bmll64 {<HSV!}===
 +
<pre>
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Wobble mll64/mll65                (70, EcoRI/BamHI
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Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L)
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Product is pBca9523-Bmll64        {<HSV!}
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----
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mll64    Forward wobble for {<HSV!} (Bmll64)
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CCATAGAATTCATGAGATCTCAGCCGGAGCTCGCACCAGAAGACC
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mll65    Reverse wobble for {<HSV!} (Bmll64)
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CGTTAGGATCCttaGCAGTCTTCCGGGTCTTCTGGTGCGAGC
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</pre>
  
 
===References===
 
===References===

Revision as of 22:35, 15 July 2010

{Bmll64} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll64 {<HSV!}

Wobble mll64/mll65                (70, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll64         {<HSV!}
----
mll64    Forward wobble for {<HSV!} (Bmll64)
CCATAGAATTCATGAGATCTCAGCCGGAGCTCGCACCAGAAGACC
mll65    Reverse wobble for {<HSV!} (Bmll64)
CGTTAGGATCCttaGCAGTCTTCCGGGTCTTCTGGTGCGAGC

References