Difference between revisions of "Part:BBa J70608"
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This is a RFC12 assembly of BBa_J70592 and BBa_J70591 so that the end user does not need to assemble the RBS into the construct every time. If you need more control over either the promoter or the RBS do not use this part. | This is a RFC12 assembly of BBa_J70592 and BBa_J70591 so that the end user does not need to assemble the RBS into the construct every time. If you need more control over either the promoter or the RBS do not use this part. | ||
− | Please note that the assembly scar as of 7/13/10 shown below is INCORRECT. The provided scar is an RFC10 scar and has sequence TACTAGAG, the correct scar is the RFC12 scar with sequence GCTAGT. | + | Please note that the assembly scar as of 7/13/10 shown below is INCORRECT. The provided scar is an RFC10 scar and has sequence TACTAGAG, the correct scar is the RFC12 scar with sequence '''GCTAGT'''. |
Latest revision as of 14:49, 13 July 2010
RFC12 Constitutive Promoter and a RBS
This is a RFC12 assembly of BBa_J70592 and BBa_J70591 so that the end user does not need to assemble the RBS into the construct every time. If you need more control over either the promoter or the RBS do not use this part.
Please note that the assembly scar as of 7/13/10 shown below is INCORRECT. The provided scar is an RFC10 scar and has sequence TACTAGAG, the correct scar is the RFC12 scar with sequence GCTAGT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]