Difference between revisions of "Part:BBa K398504:Design"

 
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<partinfo>BBa_K398054 short</partinfo>
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<partinfo>BBa_K398504 short</partinfo>
  
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<partinfo>BBa_K398054 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K398504 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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Beware of an additional XbaI site between J23100 and J61100. It's presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI).
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Beware of an additional XbaI site between J23100 and J61127. Its presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI).
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===Source===
 
===Source===

Latest revision as of 21:42, 12 July 2010

J23100 J61127 E0040 B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

Beware of an additional XbaI site between J23100 and J61127. Its presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI).

Source

iGEM 2010 distribution plates.

References