Difference between revisions of "Part:BBa K398500:Design"

 
(Design Notes)
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<partinfo>BBa_K398050 short</partinfo>
 
<partinfo>BBa_K398050 short</partinfo>
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===Design Notes===
 
===Design Notes===
Beware of an additional XbaI site between J23100 and J61100.  
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Beware of an additional XbaI site between J23100 and J61100. Its presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI).
 
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===Source===
 
===Source===

Revision as of 21:36, 12 July 2010

No part name specified with partinfo tag.

No part name specified with partinfo tag.


Design Notes

Beware of an additional XbaI site between J23100 and J61100. Its presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI).

Source

iGEM 2010 BioBrick plates.

References