Difference between revisions of "Part:BBa K398503:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K398053 short</partinfo> | <partinfo>BBa_K398053 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Beware of an additional XbaI site between J23100 and J61100. It's presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is | + | Beware of an additional XbaI site between J23100 and J61100. It's presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI). |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 21:32, 12 July 2010
No part name specified with partinfo tag.
No part name specified with partinfo tag.
Design Notes
Beware of an additional XbaI site between J23100 and J61100. It's presence facilitates promoter swapping. Thus, utilizing this construct as back insert (XbaI, PstI) will cause the loss of J23100. The construct is forward insert friendly (EcoRI, SpeI).
Source
iGEM 2010 distribution plates.