Difference between revisions of "Part:BBa K343003"

 
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<partinfo>BBa_K343003 short</partinfo>
 
<partinfo>BBa_K343003 short</partinfo>
  
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Sensory Rhodopsin II bluelight receptor fused to its transducer, HtrII, with a 27 BP linker region.  This is fused with E.Coli's chemotaxis protein Tsr, so that the protein effectively couples the input from the receptor to chemotaxis. The fusion between HtrII and Tsr is an M-fusion in the HAMP domain, which is supposed to give it maximum activity. We deduced the sequence from the instructions given by Jung, Spudich E, Trivedi and Spudich J in the article: An Archaeal Photosignal-Transducing Module Mediates Phototaxis in Escherichia coli. (1)
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:39, 2 July 2010

NpSopII-NpHtrII-StTar (M-fusion)

Sensory Rhodopsin II bluelight receptor fused to its transducer, HtrII, with a 27 BP linker region. This is fused with E.Coli's chemotaxis protein Tsr, so that the protein effectively couples the input from the receptor to chemotaxis. The fusion between HtrII and Tsr is an M-fusion in the HAMP domain, which is supposed to give it maximum activity. We deduced the sequence from the instructions given by Jung, Spudich E, Trivedi and Spudich J in the article: An Archaeal Photosignal-Transducing Module Mediates Phototaxis in Escherichia coli. (1)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1783
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 331
    Illegal AgeI site found at 1585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1027
    Illegal BsaI.rc site found at 1300
    Illegal SapI site found at 801
    Illegal SapI.rc site found at 1801