Difference between revisions of "Part:BBa P1014"
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<partinfo>BBa_P1014 short</partinfo> | <partinfo>BBa_P1014 short</partinfo> | ||
Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155 | Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155 | ||
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| + | Conveys resistance to Genamicin at up to 25 ug/ml. It conveys low levels of resistance to Kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to Gentamicin at less than 0.3 ug/ml. Selection for this cassette carried out at 5 ug/ml will allow growth in Gentamicin, but inhibit other growth. With standard Kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance. | ||
Revision as of 14:28, 2 July 2010
gentamicin resistance cassette aph(2)
Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155
Conveys resistance to Genamicin at up to 25 ug/ml. It conveys low levels of resistance to Kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to Gentamicin at less than 0.3 ug/ml. Selection for this cassette carried out at 5 ug/ml will allow growth in Gentamicin, but inhibit other growth. With standard Kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]