Difference between revisions of "Part:BBa K249019:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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When sequencing the stock we had from 2009 our team (Lethbridge) found a RBS mutation.  We attempted to overexpress the protein and found that it would not.  We do not know if this is the same for the kits that were sent out this year or not.  We recommend that you sequence the part before use and if it has the same mutation do not use directly but mutate the mutation out.  The mutation we found is as follows:
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              Mms6 7 VF Prefix  TCCCGACTGGAAAGCGGG-NNANAGNGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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Mms6 7 VR A-Suffix (reversed)  TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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              Mms6 8 VF Prefix  TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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Mms6 8 VR A-Suffix (reversed)  TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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                          mRBS  TCAC-ACAGGAAAG----------------------------------------------

Revision as of 22:15, 28 June 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K249019

User Reviews

UNIQ48b4d453ed18b460-partinfo-00000000-QINU UNIQ48b4d453ed18b460-partinfo-00000001-QINU

When sequencing the stock we had from 2009 our team (Lethbridge) found a RBS mutation. We attempted to overexpress the protein and found that it would not. We do not know if this is the same for the kits that were sent out this year or not. We recommend that you sequence the part before use and if it has the same mutation do not use directly but mutate the mutation out. The mutation we found is as follows: 

             Mms6 7 VF Prefix   TCCCGACTGGAAAGCGGG-NNANAGNGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
Mms6 7 VR A-Suffix (reversed)   TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
             Mms6 8 VF Prefix   TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
Mms6 8 VR A-Suffix (reversed)   TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
                         mRBS   TCAC-ACAGGAAAG----------------------------------------------