Difference between revisions of "Part:BBa J70594:Design"
(→Design Notes) |
(→Design Notes) |
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Made with synthetic oligos: | Made with synthetic oligos: | ||
| − | 5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3' | + | 5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3', Tail-F |
| − | 5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3' | + | 5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3', Tail-R |
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. | Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. | ||
Latest revision as of 19:41, 18 June 2010
RFC12 TAATAA Tail Domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Made with synthetic oligos:
5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3', Tail-F 5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3', Tail-R
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
Source
Common Knowledge