Difference between revisions of "Part:BBa J70594:Design"

 
(Design Notes)
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70594 short</partinfo>
 
<partinfo>BBa_J70594 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
Made with synthetic oligos:
 
Made with synthetic oligos:
5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3'
+
 
5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3'
+
5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3'
 +
5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3'
  
 
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
 
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
 
 
 
  
 
===Source===
 
===Source===

Revision as of 19:41, 18 June 2010

RFC12 TAATAA Tail Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made with synthetic oligos:

5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3'
5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3'

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

Common Knowledge

References