Difference between revisions of "Part:BBa J70592:Design"

 
(→‎Design Notes)
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70592 short</partinfo>
 
<partinfo>BBa_J70592 short</partinfo>
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Designed with synthetic oligos:
 
Designed with synthetic oligos:
  
−
5' AATTC GCGGCGC T ACTAGT TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC GCTAGC A GCGGCCG CTGCA 3', Prom-F
+
5' AATTC GCGGCGC T ACTAGT TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC GCTAGC A GCGGCCG CTGCA 3', Prom-F
−
5' GCGGCCGCTGCTAGC GCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAA ACTAGTAGCGCCGCG 3', Prom-R
+
 +
5' GCGGCCGCTGCTAGC GCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAA ACTAGTAGCGCCGCG 3', Prom-R
 +
 
 
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
 
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
−
 
−
 
  
 
===Source===
 
===Source===

Revision as of 18:08, 18 June 2010

RFC12 Constitutive Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with synthetic oligos: 

5' AATTC GCGGCGC T ACTAGT TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC GCTAGC A GCGGCCG CTGCA 3', Prom-F

5' GCGGCCGCTGCTAGC GCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAA ACTAGTAGCGCCGCG 3', Prom-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

BBa_J23100

References