Difference between revisions of "Part:BBa K416000"

Revision as of 18:51, 16 June 2010

Aga2 CDS Responsible for Yeast Surface Display

In order to extracellularly display proteins on the yeast cell surface, the protein is fused to this Aga2 protein via a flexible linker. Once secreted, the Aga2 protein binds to the Aga1 protein on the yeast surface, anchoring the fusion onto the cell surface. Because the Aga2 protein must bind Aga1 to display, the chassis yeast strain must also express Aga1.

The STOP codon at the end of the gene was removed from this CDS to allow for both N- and C-terminus fusions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K416000 short</partinfo>
-The Aga2 protein is fused in frame with a protein that the user wants to display on the yeast cellular surface. Yeast cells constitutively expressing Aga1 (strain EBY100) secrete the Aga2 fusion protein, which associates with Aga1 through two disulfide bonds and quaternary structure to anchor Aga2 to the surface. The fused protein then is displayed on the surface of the yeast cell.
+In order to extracellularly display proteins on the yeast cell surface, the protein is fused to this Aga2 protein via a flexible linker. Once secreted, the Aga2 protein binds to the Aga1 protein on the yeast surface, anchoring the fusion onto the cell surface. Because the Aga2 protein must bind Aga1 to display, the chassis yeast strain must also express Aga1.
-The STOP codon at the end of the CDS was removed to allow for N- and C-terminus fusions.
+The STOP codon at the end of the gene was removed from this CDS to allow for both N- and C-terminus fusions.
 <!-- Add more about the biology of this part here