Difference between revisions of "Part:BBa K416000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We removed the STOP codon to allow for N- and C-terminal fusions, which have both been proven to occur efficiently. | + | We have removed the STOP codon of this part to allow for N- and C-terminal Aga2 fusions, which have both been proven to occur efficiently (Refer to Wang, z. ''et. al''. : '''Protein Engineering, Design & Selection''', Vol. 18 No. 7 pp. 337-343, 2005. |
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===Source=== | ===Source=== | ||
Revision as of 18:35, 16 June 2010
Aga2 CDS Responsible for Yeast Surface Display
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have removed the STOP codon of this part to allow for N- and C-terminal Aga2 fusions, which have both been proven to occur efficiently (Refer to Wang, z. et. al. : Protein Engineering, Design & Selection, Vol. 18 No. 7 pp. 337-343, 2005.
Source
This parts was biobricked from the pCTCON2 plasmid which was sent to us by Dr. D. K. Wittrup at MIT.