Difference between revisions of "Part:BBa M33353:Design"

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===Source===
 
===Source===
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Made 1 silent mutations for XbaI, to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA and added a strong ribosome binding site (BBa_B0034).
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This is a genomic sequence from Pseudomonas putida. The was obtained from GenBank (Y18245.1; http://www.ncbi.nlm.nih.gov/nuccore/4914628).
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===References===
 
===References===

Revision as of 21:16, 6 June 2010

CDS for TodT protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 175
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction sites for EcorI, XbaI, SpeI, and PstI were removed from the sequence.


Source

Made 1 silent mutations for XbaI, to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA and added a strong ribosome binding site (BBa_B0034).

References

Lacal, Jesus, Andreas Busch, Marı´a-Eugenia Guazzaroni, Tino Krell, and Juan L. Ramos. “The TodS–TodT two-component regulatory system recognizes a wide range of effectors and works with DNA-bending proteins.” Proceedings of the National Academy of Sciences 103, no. 21 (2006): 8191-8196. http://www.pnas.org/content/103/21/8191.full.pdf+html (accessed June 6, 2010).

Lau, Peter C.K., Ying Wang, Ashok Patel, Diane Labbe, Helene Bergeron, Roland Brousseau, Yasuo Konishi, and Merriann Rawlings. “A bacterial basic region leucine zipper histidine kinase regulating toluenedegradation.” Proceedings of the National Academy of Sciences 94, no. 4 (1997): 1453–1458. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC19812/pdf/pq001453.pdf (accessed June 6, 2010).