Difference between revisions of "Part:BBa M33353"
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<partinfo>BBa_M33353 short</partinfo> | <partinfo>BBa_M33353 short</partinfo> | ||
Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. In response to toluene and other aromatic hydrocarbons, the TodS/TodT TCS mediate a single promoter termed pTod, which transcribes the catabolic genes involved in the toluene dioxygenase pathway. TodT is phosphorylated by the protein kinase TodS, and it can also increase the autophosphorylation activity of TodS. Once TodT is activated, it binds to a site on the pTod promoter approximately 107 base pairs upstream from the RNA polymerase-binding site. DNA bending caused by integration host factor (IHF) enables contact between TodT and RNA polymerase, initiating transcription. | Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. In response to toluene and other aromatic hydrocarbons, the TodS/TodT TCS mediate a single promoter termed pTod, which transcribes the catabolic genes involved in the toluene dioxygenase pathway. TodT is phosphorylated by the protein kinase TodS, and it can also increase the autophosphorylation activity of TodS. Once TodT is activated, it binds to a site on the pTod promoter approximately 107 base pairs upstream from the RNA polymerase-binding site. DNA bending caused by integration host factor (IHF) enables contact between TodT and RNA polymerase, initiating transcription. | ||
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This part should be used in conjunction with BBa_M33351, the Ptod promoter, and BBa_M33350/BBa_M33352, the todS protein sequence. | This part should be used in conjunction with BBa_M33351, the Ptod promoter, and BBa_M33350/BBa_M33352, the todS protein sequence. | ||
Latest revision as of 21:04, 6 June 2010
CDS for TodT protein
Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. In response to toluene and other aromatic hydrocarbons, the TodS/TodT TCS mediate a single promoter termed pTod, which transcribes the catabolic genes involved in the toluene dioxygenase pathway. TodT is phosphorylated by the protein kinase TodS, and it can also increase the autophosphorylation activity of TodS. Once TodT is activated, it binds to a site on the pTod promoter approximately 107 base pairs upstream from the RNA polymerase-binding site. DNA bending caused by integration host factor (IHF) enables contact between TodT and RNA polymerase, initiating transcription.
This part should be used in conjunction with BBa_M33351, the Ptod promoter, and BBa_M33350/BBa_M33352, the todS protein sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 175
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]