Difference between revisions of "Part:BBa J92005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Had to make silent mutations at 322, 823, 2137 to get rid of PstI site. | |
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===Source=== | ===Source=== | ||
Latest revision as of 05:06, 31 May 2010
M. Ruminantium RNA polymerase Subunit A'
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 263
Illegal NotI site found at 890
Illegal NotI site found at 2393
Illegal NotI site found at 2534 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 348
Illegal BglII site found at 807
Illegal BglII site found at 1095
Illegal BglII site found at 2616
Illegal BamHI site found at 1002
Illegal BamHI site found at 2502 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 873
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Had to make silent mutations at 322, 823, 2137 to get rid of PstI site.
Source
This comes from a genomic sequence