Difference between revisions of "Part:BBa J18932"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J18932 short</partinfo> | <partinfo>BBa_J18932 short</partinfo> | ||
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* bright and photo-stable | * bright and photo-stable | ||
| + | '''Degradation issues:''' | ||
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| + | * Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli | ||
| + | * SDS treatment or boiling can hydrolyze the chromophore position at F//MYG splitting the protein in half | ||
Revision as of 18:54, 26 January 2010
mCherry RFP
Red fluorescent protein derived from DsRed.
Advantages:
- fast folding and maturation
- bright and photo-stable
Degradation issues:
- Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli
- SDS treatment or boiling can hydrolyze the chromophore position at F//MYG splitting the protein in half
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]