Difference between revisions of "Part:BBa J18924"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J18924 short</partinfo> | <partinfo>BBa_J18924 short</partinfo> | ||
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* E34(I): Kd = 6.1 nM = J18923 | * E34(I): Kd = 6.1 nM = J18923 | ||
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* E34(V): Kd = 41 nM | * E34(V): Kd = 41 nM | ||
* E34(N): Kd = 810 nM | * E34(N): Kd = 810 nM | ||
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| + | (CRG-internal ID: rg0112) | ||
== References == | == References == | ||
Latest revision as of 18:10, 26 January 2010
R34 heterodimerizing leucine zipper
Engineered Leu zipper based on VBP B-ZIP domain that forms medium-weak parallel heterodimers with variants E34(X). This zipper corresponds to RR34(A) in Acharya et al and R34 in Bashor et al. Bashor et al. used the three different pairs to tune the affinity of their MAPK feedback device.
Interaction with E34(X) variants:
- E34(I): Kd = 6.1 nM = J18923
- E34(V): Kd = 41 nM
- E34(N): Kd = 810 nM
(CRG-internal ID: rg0112)
References
- Acharya et al. (2002) A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: amino acids I, V, L, N, A, and K. PMID: 12450375
- Bashor et al (2008) Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. PMID: 18339942
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]