Difference between revisions of "Part:BBa J18909:Design"

 
(→‎Design Notes)
 
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There are two HIS codons: cat and cac;
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In E.coli, cac seems preferred among highly expressed genes during exponential phase: 70%. Metabolic proteins have about even numbers of the two codons.
  
 
===Source===
 
===Source===

Latest revision as of 10:49, 15 January 2010

His tag (6xHis; E. coli - optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.


There are two HIS codons: cat and cac; In E.coli, cac seems preferred among highly expressed genes during exponential phase: 70%. Metabolic proteins have about even numbers of the two codons.

Source

Synthetic DNA

References