Difference between revisions of "Part:BBa K5248055"

Revision as of 15:35, 1 December 2024

pACYCDuet-1-mCherry-mchI（6his)-EGFP-mchB(strep)

We inoculated 100 μL of each predator strain's overnight concentrated culture on LB agar plates, covered with soft LB agar containing prey strains. For the mcmA and mchB antimicrobial peptide expression plasmids, the prey strain was Escherichia coli DH5α, and the predator strains were E. coli Nissle 1917 DE3 and BL21 DE3. In this experiment, the first two plates showed predators expressing mcmA and mchB, with the prey sensitive to these antimicrobial peptides.

< img src="https://static.igem.wiki/teams/5248/wet-lab/results/59.webp">

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 143
Illegal PstI site found at 369
Illegal PstI site found at 378
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 143
Illegal NheI site found at 589
Illegal PstI site found at 369
Illegal PstI site found at 378
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 143
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 143
Illegal PstI site found at 369
Illegal PstI site found at 378
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 143
Illegal PstI site found at 369
Illegal PstI site found at 378
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5248055 short</partinfo>
+We inoculated 100 μL of each predator strain's overnight concentrated culture on LB agar plates, covered with soft LB agar containing prey strains. For the mcmA and mchB antimicrobial peptide expression plasmids, the prey strain was Escherichia coli DH5α, and the predator strains were E. coli Nissle 1917 DE3 and BL21 DE3. In this experiment, the first two plates showed predators expressing mcmA and mchB, with the prey sensitive to these antimicrobial peptides.
+<div style="text-align: center;">
+  < img src="https://static.igem.wiki/teams/5248/wet-lab/results/59.webp">
+</div>
 <!-- Add more about the biology of this part here