Difference between revisions of "Part:BBa K5380001:Experience"
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===Applications of BBa_K5380001=== | ===Applications of BBa_K5380001=== | ||
Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014). We used this part in our project as a promoter to up regulate downstream genes in response to the presence of nitrate in our plant system. We were unable to verify its efficiency in our plant, as we were unable to verify the insertion of the part, but its efficacy is well validated in previous publications. | Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014). We used this part in our project as a promoter to up regulate downstream genes in response to the presence of nitrate in our plant system. We were unable to verify its efficiency in our plant, as we were unable to verify the insertion of the part, but its efficacy is well validated in previous publications. | ||
Revision as of 13:49, 2 October 2024
Applications of BBa_K5380001
Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014). We used this part in our project as a promoter to up regulate downstream genes in response to the presence of nitrate in our plant system. We were unable to verify its efficiency in our plant, as we were unable to verify the insertion of the part, but its efficacy is well validated in previous publications.
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