Difference between revisions of "Part:BBa K5443012"
 
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Feruloyl-CoA Synthetase activates ferulic acid by converting it into feruloyl-CoA as the third step in the vanillin biosynthesis pathway. The process involves the activation through CoA-thioesterification. We tested two FCS enzymes for this pathway step; this enzyme from Streptomyces, and also a homolog from Pseudomonas.  
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<b>Feruloyl-CoA Synthetase</b> (FCS) from <i>Streptomyces</i>, <b>FCS-Str</b>, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.
  
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https://static.igem.wiki/teams/5443/fcs-reaction.jpg
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We tested two alternate FCS enzymes for this pathway step; this enzyme from <i>Streptomyces</i>, and also a homolog from <i>Pseudomonas putida</i>, FCS-Ppu ([https://parts.igem.org/Part:BBa_K5443011 BBa_K5443011]).
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This part builds on the work of earlier iGEM teams, who contributed FCS Parts as follows: [https://parts.igem.org/Part:BBa_K4256006 BBa_K4256006].
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<html>
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<figure>
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    <img src="https://static.igem.wiki/teams/5443/fcs-reaction.jpg" style="width:600px;height:auto">
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    <figcaption>
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        <b>Figure 1. FCS-Str-catalysed conversion of ferulic acid to feruoyl-CoA.</b><br>
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        <i>This reaction represents the fourth step in our vanillin synthesis pathway. The enzyme FCS-Str converts ferulic acid into feruoyl-CoA.</i>
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    </figcaption>
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</figure>
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</html>

Latest revision as of 13:38, 2 October 2024

Feruloyl-CoA Synthetase (FCS) from Streptomyces, FCS-Str, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.

We tested two alternate FCS enzymes for this pathway step; this enzyme from Streptomyces, and also a homolog from Pseudomonas putida, FCS-Ppu (BBa_K5443011).

This part builds on the work of earlier iGEM teams, who contributed FCS Parts as follows: BBa_K4256006.


Figure 1. FCS-Str-catalysed conversion of ferulic acid to feruoyl-CoA.
This reaction represents the fourth step in our vanillin synthesis pathway. The enzyme FCS-Str converts ferulic acid into feruoyl-CoA.