Difference between revisions of "Part:BBa K5115027"

 
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===Usage and Biology===
 
===Usage and Biology===
CsoSCA encodes the β-carbonic anhydrase enzyme, which is not necessary in our design, therefore removed from the csoS operon [https://parts.igem.org/Part:BBa_K5115034 BBa_K5115034], while other subunits will still be used to generate a synthetic α-carboxysome shell in ''E. coli''.
+
The csoS3 encodes the β-carbonic anhydrase enzyme, which is not necessary in our design, therefore removed from the csoS operon [https://parts.igem.org/Part:BBa_K5115034 BBa_K5115034], while other subunits will still be used to generate a synthetic α-carboxysome shell in ''E. coli''.
  
 
===Characterization===
 
===Characterization===

Latest revision as of 13:21, 2 October 2024


csoS3

contributed by Fudan iGEM 2024

Introduction

The csoS3 is a subunit of the original csoS operon.

Usage and Biology

The csoS3 encodes the β-carbonic anhydrase enzyme, which is not necessary in our design, therefore removed from the csoS operon BBa_K5115034, while other subunits will still be used to generate a synthetic α-carboxysome shell in E. coli.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


References