Difference between revisions of "Part:BBa K5443011"
 
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<b>Feruloyl-CoA Synthetase</b> from <i>Pseudomonas putida</i>, <b>FSC-Ppu</b>, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.
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<b>Feruloyl-CoA Synthetase</b> from <i>Pseudomonas putida</i>, <b>FCS-Ppu</b>, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.
  
 
We tested two alternate FCS enzymes for this pathway step; this enzyme from <i>Pseudomonas</i>, and also a homolog from <i>Streptomyces</i> ([https://parts.igem.org/BBa_K5443012 BBa_K5443012]).
 
We tested two alternate FCS enzymes for this pathway step; this enzyme from <i>Pseudomonas</i>, and also a homolog from <i>Streptomyces</i> ([https://parts.igem.org/BBa_K5443012 BBa_K5443012]).

Latest revision as of 13:19, 2 October 2024

Feruloyl-CoA Synthetase from Pseudomonas putida, FCS-Ppu, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.

We tested two alternate FCS enzymes for this pathway step; this enzyme from Pseudomonas, and also a homolog from Streptomyces (BBa_K5443012).

This part builds on the work of earlier iGEM teams, who contributed FCS Parts as follows: BBa_K1129002

Figure 1. FCS-Ppu-catalysed conversion of ferulic acid to feruoyl-CoA.
This reaction represents the fourth step in our vanillin synthesis pathway. The enzyme FCS-Ppu converts ferulic acid into feruoyl-CoA.