Difference between revisions of "Part:BBa K5443030"
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<img src="https://static.igem.wiki/teams/5443/pmq3c-map-v2.jpg" style="width:600px;height:auto"> | <img src="https://static.igem.wiki/teams/5443/pmq3c-map-v2.jpg" style="width:600px;height:auto"> | ||
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| − | <b>Figure 1. Structure of composite part K5443028: Insert DNA in Vanillin biosynthetic plasmid | + | <b>Figure 1. Structure of composite part K5443028: Insert DNA in Vanillin biosynthetic plasmid pMQ3C </b><br> |
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Revision as of 13:15, 2 October 2024
This composite part consists of five genes (ECH-Ppu, FCS-Ppu, COMT-ara, C3H-HpaBC and TAL-Sam8), each with a strong RBS attached, cloned into an expression vector (pUS250) to make the construct pMQ3C. The RBS used are all slightly different variants of B0034, designed to be of predicted strength 10,000 (arbitrary units) using the DeNovoDNA website RBS calculator. The genes are organised into a two-gene cluster (ECH-Ppu + FCS-Ppu) and three-gene cluster (COMT-ara, C3H-HpaBC, TAL-Sam8), with each cluster under the control of its own cumate-inducible promoter. The two cumate promoter sequences are intended to be functionally identical but the non-essential sequence elements have been modified in the second promoter to minimise plasmid mutations due to homologous recombination.
