Difference between revisions of "Part:BBa K5443031"
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     <img src="https://static.igem.wiki/teams/5443/pmq3d-map.jpg" style="width:600px;height:auto">
 
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     <figcaption> Figure 1. Structure of composite part K5443031: Insert DNA in Vanillin biosynthetic plasmid pMQ3D </b><br>
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        <b>Figure 1. LCMS analysis of compounds produced using pMQ3C-11.</b><br>
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        <i>The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11.</i>
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Revision as of 13:13, 2 October 2024

This composite part consists of five genes (ECH-Str, FCS-Str, COMT-ara, C3H-Sam5 and TAL-Sam8), each with a strong RBS attached, cloned into an expression vector (pUS250) to make the construct pMQ3C. The RBS used are all slightly different variants of B0034, designed to be of predicted strength 10,000 (arbitrary units) using the DeNovoDNA website RBS calculator. The genes are organised into a two-gene cluster (ECH-Str, FCS-Str) and three-gene cluster (COMT-ara, C3H-Sam5 and TAL-Sam8), with each cluster under the control of its own cumate-inducible promoter. The two cumate promoter sequences are intended to be functionally identical but the non-essential sequence elements have been modified in the second promoter to minimise plasmid mutations due to homologous recombination.


Figure 1. Structure of composite part K5443031: Insert DNA in Vanillin biosynthetic plasmid pMQ3D