Difference between revisions of "Part:BBa K5443011:Experience"
 
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Our LC-MS analysis showed the presence of vanillin (0.2 ppm) in reactions, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including FCS-Ppu . See LC-MS data below (Fig. 1).
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===Applications of BBa_K5443011===
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We showed that the FCS-Ppu enzyme from <i>Pseudomonas putida</i> was functional in <i>E. coli</i> as part of a vanillin biosynthetic pathway. While we were unable to use LC-MS to detect feruoyl-CoA directly (due to issues procuring an analytial standard), we were able to successfully detect all other metabolites in the pathway, including the final desired product of vanillin (Fig. 1).
  
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https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg
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As functional FCS-Ppu is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.
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<figure>
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    <img src="https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg" style="width:600px;height:auto">
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    <figcaption>
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        <b>Figure 1. LCMS analysis of compounds produced using pMQ3C-11.</b><br>
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        <i>The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including FCS-Ppu.</i>
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    </figcaption>
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</figure>
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Latest revision as of 13:09, 2 October 2024

Applications of BBa_K5443011

We showed that the FCS-Ppu enzyme from Pseudomonas putida was functional in E. coli as part of a vanillin biosynthetic pathway. While we were unable to use LC-MS to detect feruoyl-CoA directly (due to issues procuring an analytial standard), we were able to successfully detect all other metabolites in the pathway, including the final desired product of vanillin (Fig. 1).

As functional FCS-Ppu is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.

Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including FCS-Ppu.