Difference between revisions of "Part:BBa K5291031"
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We have known from papers related to PE degradation that during this process a huge amount of CO<sub>2</sub> will be released, which is hard to solute in the water and exists risks of exceeding the absorption limit of mangroves. Therefore, we introduce this system to address the problem of CO<sub>2</sub>. The gene <i>PAO102</i> is native in <i>Pseudomonas aeruginosa</i> and can speed up the conversion between CO<sub>2</sub> and bicarbonate. And the promoter pS is verified that it is able to come into effect in <i>P. aeruginosa</i>.<br><br> | We have known from papers related to PE degradation that during this process a huge amount of CO<sub>2</sub> will be released, which is hard to solute in the water and exists risks of exceeding the absorption limit of mangroves. Therefore, we introduce this system to address the problem of CO<sub>2</sub>. The gene <i>PAO102</i> is native in <i>Pseudomonas aeruginosa</i> and can speed up the conversion between CO<sub>2</sub> and bicarbonate. And the promoter pS is verified that it is able to come into effect in <i>P. aeruginosa</i>.<br><br> | ||
| − | <html><img width = " | + | <html><img width = "490" src="https://static.igem.wiki/teams/5291/images/part-cr/pao102-schema-correct.png" /></html><br> |
<b>Fig.1 The schema of pAB1-pS-<i>PAO102</i>.</b><br><br> | <b>Fig.1 The schema of pAB1-pS-<i>PAO102</i>.</b><br><br> | ||
We apply the method of <i>PAO102</i> high copies, so that CO<sub>2</sub> produced by engineered <i>P. aeruginosa</i> can be swiftly conversed to bicarbonate, easily transporting in the biofilm.<br> | We apply the method of <i>PAO102</i> high copies, so that CO<sub>2</sub> produced by engineered <i>P. aeruginosa</i> can be swiftly conversed to bicarbonate, easily transporting in the biofilm.<br> | ||
| − | <html><img width = " | + | <html><img width = "200" src="https://static.igem.wiki/teams/5291/images/part-cr/pao102-colonypcr.png" /></html> |
| − | <html><img width = " | + | <html><img width = "580" src="https://static.igem.wiki/teams/5291/images/part-cr/3.png" /></html><br> |
<b>Fig.2 The AGE figure of <i>PAO102</i> colony PCR and the map of the plasmid.</b><br><br> | <b>Fig.2 The AGE figure of <i>PAO102</i> colony PCR and the map of the plasmid.</b><br><br> | ||
However, there is a great pity that we have not finished the verification of the function due to rush time.<br><br> | However, there is a great pity that we have not finished the verification of the function due to rush time.<br><br> | ||
Latest revision as of 13:06, 2 October 2024
pAB1-pS-PAO102
A system used to deal with CO2 released from Pseudomonas aeruginosa during PE degradation.
Usage and Biology
We have known from papers related to PE degradation that during this process a huge amount of CO2 will be released, which is hard to solute in the water and exists risks of exceeding the absorption limit of mangroves. Therefore, we introduce this system to address the problem of CO2. The gene PAO102 is native in Pseudomonas aeruginosa and can speed up the conversion between CO2 and bicarbonate. And the promoter pS is verified that it is able to come into effect in P. aeruginosa.
Fig.1 The schema of pAB1-pS-PAO102.
We apply the method of PAO102 high copies, so that CO2 produced by engineered P. aeruginosa can be swiftly conversed to bicarbonate, easily transporting in the biofilm.
Fig.2 The AGE figure of PAO102 colony PCR and the map of the plasmid.
However, there is a great pity that we have not finished the verification of the function due to rush time.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 110
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 110
Illegal NotI site found at 291 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 110
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 110
- 1000COMPATIBLE WITH RFC[1000]