Difference between revisions of "Part:BBa K5291022"
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We select PcW as the replacing promoter of <i>hok</i>/<i>mok</i> through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of <i>sok</i> is stronger than <i> hok</i>/<i>mok</i> when there is citrate in the environment, preventing cell death in advanced.<br><br> | We select PcW as the replacing promoter of <i>hok</i>/<i>mok</i> through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of <i>sok</i> is stronger than <i> hok</i>/<i>mok</i> when there is citrate in the environment, preventing cell death in advanced.<br><br> | ||
| − | <html><img width = " | + | <html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html> |
<br><b>Fig.1 The schema of <i>hok</i>/<i>sok</i> system. The promoter of <i>hok</i>/<i>mok</i> is PcW.</b><br><br> | <br><b>Fig.1 The schema of <i>hok</i>/<i>sok</i> system. The promoter of <i>hok</i>/<i>mok</i> is PcW.</b><br><br> | ||
We produce the <i>hok</i>/<i>sok</i> system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037. | We produce the <i>hok</i>/<i>sok</i> system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037. | ||
Latest revision as of 12:59, 2 October 2024
PcW
A common weak constitutive promoter in Pseudomonas aeruginosa.
Usage and Biology
We select PcW as the replacing promoter of hok/mok through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of sok is stronger than hok/mok when there is citrate in the environment, preventing cell death in advanced.
Fig.1 The schema of hok/sok system. The promoter of hok/mok is PcW.
We produce the hok/sok system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]