Difference between revisions of "Part:BBa K5051000:Design"

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===References===
 
===References===
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1. Xue, S., Liu, G., Chi, Z., Gao, Z., Hu, Z., & Chi, Z. (2020). Genetic evidences for the core biosynthesis pathway, regulation, transport and secretion of liamocins in yeast-like fungal cells. Biochemical Journal, 477(5), 887–903. https://doi.org/10.1042/bcj20190922
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<p>1. Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., Bridgland, A., Meyer, C., Kohl, S. a. A., Ballard, A. J., Cowie, A., Romera-Paredes, B., Nikolov, S., Jain, R., Adler, J., . . . Hassabis, D. (2021). Highly accurate protein structure prediction with AlphaFold. Nature, 596(7873), 583–589. https://doi.org/10.1038/s41586-021-03819-2</p>
 +
<p>2. Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: making protein folding accessible to all. Nature Methods, 19(6), 679–682. https://doi.org/10.1038/s41592-022-01488-1</p>
 +
<p>3. Xue, S., Liu, G., Chi, Z., Gao, Z., Hu, Z., & Chi, Z. (2020). Genetic evidences for the core biosynthesis pathway, regulation, transport and secretion of liamocins in yeast-like fungal cells. Biochemical Journal, 477(5), 887–903. https://doi.org/10.1042/bcj20190922</p>

Latest revision as of 12:58, 2 October 2024


PKS1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1012
    Illegal BglII site found at 1504
    Illegal BglII site found at 4192
    Illegal BglII site found at 5371
    Illegal BglII site found at 5677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2939
    Illegal SapI.rc site found at 5579


Design Notes

It was codon optimized for Saccharomyces cerevisiae


Source

The PKS1 gene is from Aureobasidium pullulans EXF-150 genomic sequence

References

1. Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., Bridgland, A., Meyer, C., Kohl, S. a. A., Ballard, A. J., Cowie, A., Romera-Paredes, B., Nikolov, S., Jain, R., Adler, J., . . . Hassabis, D. (2021). Highly accurate protein structure prediction with AlphaFold. Nature, 596(7873), 583–589. https://doi.org/10.1038/s41586-021-03819-2

2. Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: making protein folding accessible to all. Nature Methods, 19(6), 679–682. https://doi.org/10.1038/s41592-022-01488-1

3. Xue, S., Liu, G., Chi, Z., Gao, Z., Hu, Z., & Chi, Z. (2020). Genetic evidences for the core biosynthesis pathway, regulation, transport and secretion of liamocins in yeast-like fungal cells. Biochemical Journal, 477(5), 887–903. https://doi.org/10.1042/bcj20190922