Difference between revisions of "Part:BBa K5051000"
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The PKS1 gene from Aureobasidium pullulans EXF-150 encodes a highly reduced polyketide synthase (HR-PKS) that plays a vital role in synthesising the fatty acid tail of liamocin. This enzyme is involved in the biosynthesis 3,5-dihydroxydecanoic acid chain (fatty acid part) of Liamocin (Xue et al., 2020). The product of the PKS1 gene is the main building block of the lipid moiety of liamocin. This polyketide synthase is a multidomain enzyme that coordinates a series of reactions that are critical to producing the unique dihydroxy fatty acid chains characteristic of the liamocin molecule. | The PKS1 gene from Aureobasidium pullulans EXF-150 encodes a highly reduced polyketide synthase (HR-PKS) that plays a vital role in synthesising the fatty acid tail of liamocin. This enzyme is involved in the biosynthesis 3,5-dihydroxydecanoic acid chain (fatty acid part) of Liamocin (Xue et al., 2020). The product of the PKS1 gene is the main building block of the lipid moiety of liamocin. This polyketide synthase is a multidomain enzyme that coordinates a series of reactions that are critical to producing the unique dihydroxy fatty acid chains characteristic of the liamocin molecule. | ||
| + | https://static.igem.wiki/teams/5051/parts/parts1.png | ||
| + | Fig 1. Structure predicted by ColabFold, coloured by pLDDT (Jumper et al., 2021; Mirdita et al., 2022) | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:57, 2 October 2024
PKS1
The PKS1 gene from Aureobasidium pullulans EXF-150 encodes a highly reduced polyketide synthase (HR-PKS) that plays a vital role in synthesising the fatty acid tail of liamocin. This enzyme is involved in the biosynthesis 3,5-dihydroxydecanoic acid chain (fatty acid part) of Liamocin (Xue et al., 2020). The product of the PKS1 gene is the main building block of the lipid moiety of liamocin. This polyketide synthase is a multidomain enzyme that coordinates a series of reactions that are critical to producing the unique dihydroxy fatty acid chains characteristic of the liamocin molecule.
Fig 1. Structure predicted by ColabFold, coloured by pLDDT (Jumper et al., 2021; Mirdita et al., 2022)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1012
Illegal BglII site found at 1504
Illegal BglII site found at 4192
Illegal BglII site found at 5371
Illegal BglII site found at 5677 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2939
Illegal SapI.rc site found at 5579