Difference between revisions of "Part:BBa K5443013:Experience"
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Our LC-MS analysis showed the presence of vanillin (0.2 ppm) in reactions, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including ECH-Ppu . See LC-MS data below (Fig. 1).
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===Applications of BBa_K5443013===
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We showed that the ECH-Ppu enzyme from <i>Pseudomonas putida</i> was functional in <i>E. coli</i> as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).
  
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https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg
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As functional Ech-Ppu is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.
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<figure>
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    <img src="https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg" style="width:600px;height:auto">
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    <figcaption>
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        <b>Figure 1. LCMS analysis of compounds produced using pMQ3C-11.</b><br>
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        <i>The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including ECH-Ppu.</i>
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    </figcaption>
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</figure>
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Revision as of 12:44, 2 October 2024

Applications of BBa_K5443013

We showed that the ECH-Ppu enzyme from Pseudomonas putida was functional in E. coli as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).

As functional Ech-Ppu is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.


Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including ECH-Ppu.