Difference between revisions of "Part:BBa K5291010"
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The gene <i>hok</i>/<i>mok</i> from <i>hok</i>/<i>sok</i> system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And <i>sok</i>, the gene also in <i>hok</i>/<i>sok</i> system, can encode a kind of antisense RNA to specifically bind to <i>hok</i> mRNA, repressing the expression of toxin.<br><br> | The gene <i>hok</i>/<i>mok</i> from <i>hok</i>/<i>sok</i> system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And <i>sok</i>, the gene also in <i>hok</i>/<i>sok</i> system, can encode a kind of antisense RNA to specifically bind to <i>hok</i> mRNA, repressing the expression of toxin.<br><br> | ||
| − | <html><img width = " | + | <html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html><br> |
<b>Fig.1 The schema of <i>hok</i>/<i>mok</i> (the gene on the right)</b><br><br> | <b>Fig.1 The schema of <i>hok</i>/<i>mok</i> (the gene on the right)</b><br><br> | ||
| − | <html><img width = " | + | <html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-normal.png" /></html><br><br> |
<b>Fig.2 The mechanism of <i>hok</i>/<i>sok</i> system</b><br> | <b>Fig.2 The mechanism of <i>hok</i>/<i>sok</i> system</b><br> | ||
| − | <html><img width = " | + | <html><img width = "300" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-pcr-arm2.png" /></html><br> |
<b>Fig.3 The AGE figure of <i>hok</i>/<i>sok</i></b><br><br> | <b>Fig.3 The AGE figure of <i>hok</i>/<i>sok</i></b><br><br> | ||
For convenience, we set <i>hok</i>/<i>mok</i> and <i>sok</i> as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of <i>hok</i>/<i>mok</i>.<br><br> | For convenience, we set <i>hok</i>/<i>mok</i> and <i>sok</i> as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of <i>hok</i>/<i>mok</i>.<br><br> | ||
Latest revision as of 12:43, 2 October 2024
hok/mok
The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by sok antisense RNA.
Usage and Biology
The gene hok/mok from hok/sok system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And sok, the gene also in hok/sok system, can encode a kind of antisense RNA to specifically bind to hok mRNA, repressing the expression of toxin.
Fig.1 The schema of hok/mok (the gene on the right)
Fig.2 The mechanism of hok/sok system
Fig.3 The AGE figure of hok/sok
For convenience, we set hok/mok and sok as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of hok/mok.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]