Difference between revisions of "Part:BBa K5443030:Experience"
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Our LC-MS analysis showed the presence of vanillin (0.2 ppm) in reactions, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including FCS-Ppu . See LC-MS data below (Fig. 1).  
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Our LC-MS analysis of E.coli DH10B(pMQ1C-11) cultures growing in tyrosine-enriched (0.5 g/L) LB medium showed the presence of vanillin (0.2 ppm) in reactions, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional. See LC-MS data below (Fig. 1).  
  
 
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Revision as of 12:38, 2 October 2024

Our LC-MS analysis of E.coli DH10B(pMQ1C-11) cultures growing in tyrosine-enriched (0.5 g/L) LB medium showed the presence of vanillin (0.2 ppm) in reactions, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional. See LC-MS data below (Fig. 1).

Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11.