Difference between revisions of "Part:BBa K5236020"

Latest revision as of 12:22, 2 October 2024

pET28a-pelB-BhrPETase(N205G)-6xHis

This composite part codes for the mutated BhrPETase N205G and expresses the enzyme in the extracellular space of chassis.

T7 promoter: used to regulate gene expression of recombinant proteins.

Lac operator: used to control gene expression by lactose.

RBS: the location to bind ribosome.

BhrPETase N205G: This sequence encodes for a mutated BhrPETase N205G and is derived from Escherichia coli.

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.

Fig.1 Constructed Plasmid

Fig.2 The result of pET28a-pelB-BhrPETase(N205G)-6xHis DNA sequencing

Sequence and Features

Assembly Compatibility:

@@ Line 18: / Line 18: @@
 T7 terminator: terminates transcription.
+<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/n205g-plasmid.png" width = "50%"><br></html></center>
+<center>Fig.1 Constructed Plasmid  </center>
 <center><html><img src ="https://static.igem.wiki/teams/5236/part-images/n205g.png" width = "50%"><br></html></center>
-<center>Fig.1 The result of pET28a-pelB-BhrPETase(N205G)-6xHis DNA sequencing  </center>
+<center>Fig.2 The result of pET28a-pelB-BhrPETase(N205G)-6xHis DNA sequencing  </center>
 <!-- Add more about the biology of this part here