Difference between revisions of "Part:BBa K5142043"

 
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>The partial sequence of A26L was cloned by PCR amplification from the genome of vaccinia virus (GenBank ID: JX489136.1).</span>
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<span class='h3bb'>In our project, the A26L DNA sequence was served as a homologous arm for recombination only. Thus, the A26L sequence was not necessarily to be complete cds. Considering the whole A26L sequence is too long as a homologous arm, we chose 500 bp sequence adjacent to A27L only to construct the recombinant element.</span>
 
<partinfo>BBa_K5142043 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5142043 SequenceAndFeatures</partinfo>
  

Latest revision as of 12:06, 2 October 2024


Vaccinia virus A26L (partial)

This part includes the promoter and partial coding sequence (cds) of A26L, a membrane protein of vaccinia virus (VACV). Since A26L is located at the downstream region following A27L in vaccinia virus genome, we used partial A26L sequence (500 bp) as a homologous arm for the recombination to mutate A27L in our project.

The partial sequence of A26L was cloned by PCR amplification from the genome of vaccinia virus (GenBank ID: JX489136.1).

In our project, the A26L DNA sequence was served as a homologous arm for recombination only. Thus, the A26L sequence was not necessarily to be complete cds. Considering the whole A26L sequence is too long as a homologous arm, we chose 500 bp sequence adjacent to A27L only to construct the recombinant element.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 524
    Illegal PstI site found at 538
    Illegal NotI site found at 7
    Illegal NotI site found at 531
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 524
    Illegal PstI site found at 538
  • 1000
    COMPATIBLE WITH RFC[1000]