Difference between revisions of "Part:BBa K5443010:Experience"

 
 
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===Applications of BBa_K5443010===
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We showed that the COMT enzyme from <i>Synechocystis PCC6714</i> was functional in <i>E. coli</i> as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of ferulic acid (0.1 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).
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    <img src="https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg" style="width:600px;height:auto">
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    <figcaption>
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        <b>Figure 1. LCMS analysis of compounds produced using pMQ3C-11.</b><br>
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        <i>The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including COMT.</i>
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    </figcaption>
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__NOTOC__
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
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===Applications of BBa_K5443010===
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 11:58, 2 October 2024

Applications of BBa_K5443010

We showed that the COMT enzyme from Synechocystis PCC6714 was functional in E. coli as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of ferulic acid (0.1 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).


Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including COMT.



User Reviews

UNIQ7be72ffe138ca84d-partinfo-00000001-QINU UNIQ7be72ffe138ca84d-partinfo-00000002-QINU