Difference between revisions of "Part:BBa K5127006:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.
+
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in <i>E. coli</i>.
  
  
  
 
===Source===
 
===Source===
−
Genome of Variovorax species
+
Genome of <i>Variovorax species</i>.
  
  

Latest revision as of 11:56, 2 October 2024


iadCDE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2355
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2355
    Illegal NheI site found at 677
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2355
    Illegal BglII site found at 2364
    Illegal BglII site found at 2416
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2355
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2355
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
    Illegal NgoMIV site found at 623
    Illegal NgoMIV site found at 1614
    Illegal AgeI site found at 387
    Illegal AgeI site found at 1416
    Illegal AgeI site found at 1857
    Illegal AgeI site found at 2280
    Illegal AgeI site found at 2714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.


Source

Genome of Variovorax species.


References

Conway, J. M., Walton, W. G., Salas-González, I., Law, T. F., Lindberg, C. A., Crook, L. E., Kosina, S. M., Fitzpatrick, C. R., Lietzan, A. D., Northen, T. R., Jones, C. D., Finkel, O. M., Redinbo, M. R., & Dangl, J. L. (2022). Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome. Nature Microbiology, 7(11), 1817–1833. https://doi.org/10.1038/s41564-022-01244-3

Gordon, S. A., & Weber, R. P. (1951). Colorimetric Estimation of indoleacetic Acid. Plant Physiology, 26(1), 192–195. https://doi.org/10.1104/pp.26.1.192

Salis, H. M., Mirsky, E. A. & Voigt, C. A. (2009). Automated design of synthetic ribosome binding sites to control protein expression. Nature Biotechnology, 27(10), 946–950. https://doi.org/10.1038/nbt.1568