Difference between revisions of "Part:BBa K209474"
 
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===Usage and Biology===
 
===Usage and Biology===
 
The purpose of this part is to insert stop codons in 3 different reading frames into the C-D position of an AarI fusion contruct.  The FKBP domain comes after the stop codons, and is only included to facilitate extraction and ligation of this part.
 
The purpose of this part is to insert stop codons in 3 different reading frames into the C-D position of an AarI fusion contruct.  The FKBP domain comes after the stop codons, and is only included to facilitate extraction and ligation of this part.
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===Documentation and characterization===
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This construct was used to express the DOR receptor without a C-terminal fusion, described here:
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http://2009.igem.org/Team:UCSF/NavigationPart2
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 +
  
 
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Latest revision as of 21:24, 1 November 2009

AarI C-D part, 3xSTOP-FKBP

Stop codons, flanked by AarI CD sites.


Usage and Biology

The purpose of this part is to insert stop codons in 3 different reading frames into the C-D position of an AarI fusion contruct. The FKBP domain comes after the stop codons, and is only included to facilitate extraction and ligation of this part.

Documentation and characterization

This construct was used to express the DOR receptor without a C-terminal fusion, described here:

http://2009.igem.org/Team:UCSF/NavigationPart2


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]