Difference between revisions of "Part:BBa K5184075"

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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5184075 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5184075 SequenceAndFeatures</partinfo>
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===Toxicity Verification===
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Cs1A is synthesized using the vector pET28a-G1M5-His-SUMO-Cs1A-GNA-His[Fig2B], of which is assembled using GoldenGate cloning and transformed into <i>E. coli</i> strain DH5ɑ. Colony PCR and sequencing is then carried out to verify the plasmid construct, of which is extracted and transformed into BL21(DE3) strain for expression. After IPTG induction and overnight incubation, the liquid culture is harvested and, after cell lysis, have an SDS-PAGE run. The results suggest that Cs1A had achieved soluble expression. After several unsuccessful purification attempts, the supernatant is treated directly by SUMO protease [Fig2C].
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<center><html><img src="https://static.igem.wiki/teams/5184/parts/cs1a-sumo.webp" width="600"/></html></center>
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<center><b>Fig2: (A) G1M5 tag allows secretion of the fusion protein into extracellular milieu (B) Plasmid construct pET28a-G1M5-His-SUMO-Cs1A-GNA-His (C) SDS-PAGE of supernatant and SUMO-treated supernatant, with supernatant of similarly treated supernatant of BL21(DE3) as control</b></center>
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The SUMO-digested supernatant's toxicity against ''T. urticae'' females is tested using a spraying method by Professor Huang from SCAU.
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Results from the toxicity assay suggests Cs1A to be highly toxic against ''T. urticae'', achieving an fatality of 92.73% within 72 hours.
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<center><html><img src="https://static.igem.wiki/teams/5184/parts/cs1a-lethality.webp" width="600"/></html></center>
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<center><b>Fig3: (A) ''T. urticae'' in their normal state, before being sprayed with treated supernatant (B) Dead ''T. urticae'' from spraying of treated supernatant (C) Survival plot of ''T. urticae'' being sprayed with supernatant containing Cs1A over 72 hours, CK is similarly processed supernatant of BL21(DE3), acting as a control (D) Lethality data of ''T. urticae'' being sprayed with supernatant over 24, 48, and 72 hours, CK is the similarly processed supernatant of BL21(DE3), acting as a control</b></center>
  
 
===Reference===
 
===Reference===

Revision as of 11:50, 2 October 2024


G1M5 tag-Cs1A-GNA

Abstract

This composite part is an expression box for the basic part Cs1A (BBa_K5184032), which allows the Cs1A-GNA fusion protein, with high contact and oral toxicity, to achieve soluble expression in E. coli. This composite part includes a G1M5-SUMO (BBa_K5184022) compound tag is annexed to the N-terminus of the fusion protein, which facilitates the protein's correct folding and therefore its soluble expression. GNA, Galanthus nivalis agglutinin (BBa_K1974020) is attached to the C-terminus of the venom peptide, protecting it and conducting it in and through insect guts; achieving high oral and contact toxicity.

Usage and Biology

G1M5 is the mutated, less hydrophobic version of the secretion signal peptide of the G1 cyclomaltodextrin glucanotransferase (CGtase) of Bacillus sp., which allows the extracellular secretion of the bacterial enzyme. Conduction of proteins attached by G1M5 out of the cytosol is achieved by the Sec pathway, a very common secretion system seen in all three major domains of life: arachaea, prokaryote, and eukaryotes. Once the signal peptide, in this case G1M5 is synthesized, the protein chaperon SecB binds to the preprotein (that is attached to G1M5), and transfers the preprotein to the protein translocase SecA, of which binds to the membrane bound protein conducting channel SecYEG. Once bound to the membrane, SecA binds to a molecule of ATP, of which is hydrolyzed to conduct the protein through heterotrimer complex of SecYEG. A membrane bound SPaseI then, once enough of the preprotein had been conducted through the channel, will remove the SP and allow the preprotein to fold properly into the correct protein.[1] The in vivo SUMO tags, or small ubiquitin-like modifiers, are a diverse class of ubiquitin-related proteins that allow a versatile range of operations to be carried on a tagged protein. Amongst many of these is the selective proteolysis that removes the SUMO tag from the attached protein. Its ability to be detected and removed with high precision leads to the creation of the recombinant SUMO tag: a SUMO tag that, while different enough to the wildtype SUMO tag to not be degraded within eukaryotic hosts, can be detected and have the SUMO removed by special recombinant SUMO proteases. The recombinant SUMO therefore presents themselves as precise markers for points for proteolytic digestion that can remove attached (to SUMO tag) protein domains. SUMO tags also share high level of homology with the ubiquitin, and therefore, once synthesized, folds rapidly to hide its hydrophobic cores. It is proposed that this initial folding assists in the correct folding of the attached fusion protein by supplying a great initial "kinetic push". While this can explain SUMO tag's ability to facilitate protein folding and enhance soluble expression, there are also theories proposing the SUMO tag to work as a chaperone to assists in the attached protein's folding.[2]

Cs1A is synthesized using the vector pET28a-G1M5-His-SUMO-Cs1A-GNA-His[Fig1B], of which is assembled using GoldenGate cloning and transformed into E. coli strain DH5ɑ. Colony PCR and sequencing is then carried out to verify the plasmid construct, of which is extracted and transformed into BL21(DE3) strain for expression. After IPTG induction and overnight incubation, the liquid culture is harvested and, after cell lysis, have an SDS-PAGE run. The results suggest that Cs1A had achieved soluble expression. After several unsuccessful purification attempts, the supernatant is treated directly by SUMO protease [Fig2C].

Fig2: (A) G1M5 tag allows secretion of the fusion protein into extracellular milieu (B) Plasmid construct pET28a-G1M5-His-SUMO-Cs1A-GNA-His (C) SDS-PAGE of supernatant and SUMO-treated supernatant, with supernatant of similarly treated supernatant of BL21(DE3) as control

The SUMO-digested supernatant's toxicity against T. urticae females is tested using a spraying method by Professor Huang from SCAU. Results from the toxicity assay suggests Cs1A to be highly toxic against T. urticae, achieving an fatality of 92.73% within 72 hours.

Fig3: (A) T. urticae in their normal state, before being sprayed with treated supernatant (B) Dead T. urticae from spraying of treated supernatant (C) Survival plot of T. urticae being sprayed with supernatant containing Cs1A over 72 hours, CK is similarly processed supernatant of BL21(DE3), acting as a control (D) Lethality data of T. urticae being sprayed with supernatant over 24, 48, and 72 hours, CK is the similarly processed supernatant of BL21(DE3), acting as a control

Galanthus nivalis agglutinin (GNA), a plant derived mannose-specific lectin that provides resistance to enzyme-meditated proteolytic activity in insect gastrointestinal tract is introduced and fused with the venom peptides. GNA is able to transport attached peptides across the insect gut, allowing delivery to the circulatory system. Thus, GNA allows enhanced oral and contact toxicity for the fusion proteins.[3]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Toxicity Verification

Cs1A is synthesized using the vector pET28a-G1M5-His-SUMO-Cs1A-GNA-His[Fig2B], of which is assembled using GoldenGate cloning and transformed into E. coli strain DH5ɑ. Colony PCR and sequencing is then carried out to verify the plasmid construct, of which is extracted and transformed into BL21(DE3) strain for expression. After IPTG induction and overnight incubation, the liquid culture is harvested and, after cell lysis, have an SDS-PAGE run. The results suggest that Cs1A had achieved soluble expression. After several unsuccessful purification attempts, the supernatant is treated directly by SUMO protease [Fig2C].

Fig2: (A) G1M5 tag allows secretion of the fusion protein into extracellular milieu (B) Plasmid construct pET28a-G1M5-His-SUMO-Cs1A-GNA-His (C) SDS-PAGE of supernatant and SUMO-treated supernatant, with supernatant of similarly treated supernatant of BL21(DE3) as control

The SUMO-digested supernatant's toxicity against T. urticae females is tested using a spraying method by Professor Huang from SCAU. Results from the toxicity assay suggests Cs1A to be highly toxic against T. urticae, achieving an fatality of 92.73% within 72 hours.

Fig3: (A) T. urticae in their normal state, before being sprayed with treated supernatant (B) Dead T. urticae from spraying of treated supernatant (C) Survival plot of T. urticae being sprayed with supernatant containing Cs1A over 72 hours, CK is similarly processed supernatant of BL21(DE3), acting as a control (D) Lethality data of T. urticae being sprayed with supernatant over 24, 48, and 72 hours, CK is the similarly processed supernatant of BL21(DE3), acting as a control

Reference

[1]Liu, Changjun, et al. ‘A Secretory System for Extracellular Production of Spider Neurotoxin Huwentoxin-I in Escherichia Coli’. Preparative Biochemistry & Biotechnology, vol. 53, no. 8, Sept. 2023, pp. 914–22. DOI.org (Crossref), https://doi.org/10.1080/10826068.2022.2158473 [2]Jürgen Dohmen, R. ‘SUMO Protein Modification’. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, vol. 1695, no. 1–3, Nov. 2004, pp. 113–31. DOI.org (Crossref), https://doi.org/10.1016/j.bbamcr.2004.09.021 [3]Sukiran, Nur Afiqah, et al. “Enhancing the Oral and Topical Insecticidal Efficacy of a Commercialized Spider Venom Peptide Biopesticide via Fusion to the Carrier Snowdrop Lectin ( Galanthus Nivalis Agglutinin).” Pest Management Science, vol. 79, no. 1, Jan. 2023, pp. 284–94. DOI.org (Crossref), https://doi.org/10.1002/ps.7198