Difference between revisions of "Part:BBa K5443009:Experience"
 
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We showed that the Arabidopsis COMT enzyme was functional in E.coli as part of a vanillin biosynthetic pathway, as evidenced by production of ferulic acid (0.1 ppm) (see LC-MS data from one clone pMQ3C-11 below) and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including COMT-ara.  
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Our LC-MS analysis showed the presence of ferulate (0.1 ppm) in reactions, and we also succeeded in producing a small amount of vanillin (0.2 ppm), and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including COMT-ara. See LC-MS data below (Fig. 1).
  
 
https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg
 
https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg

Latest revision as of 11:39, 2 October 2024

Our LC-MS analysis showed the presence of ferulate (0.1 ppm) in reactions, and we also succeeded in producing a small amount of vanillin (0.2 ppm), and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including COMT-ara. See LC-MS data below (Fig. 1).

pmq3c11-lcms-data-v2.jpg


UNIQ57efe08c184ca9cc-partinfo-00000000-QINU UNIQ57efe08c184ca9cc-partinfo-00000001-QINU