Difference between revisions of "Part:BBa K5443013:Experience"

Revision as of 11:32, 2 October 2024

Our LC-MS analysis showed the presence of vanillin (0.2 ppm) in reactions, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including ECH-Ppu . See LC-MS data below (Fig. 1).

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−	We showed that the ECH-Ppu ~~enzyme was functional in E~~.~~coli as part of a vanillin biosynthetic pathway, as evidenced by production of vanillin (see~~ LC-MS data ~~from one clone pMQ3C-11~~).	+	Our LC-MS analysis showed the presence of vanillin (0.2 ppm) in reactions, and thus we can conclude that the enzyme Parts in the plasmid pMQ3C-11 were all at least partially functional, including ECH-Ppu . See LC-MS data below (Fig. 1).

	https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg		https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg