Difference between revisions of "Part:BBa K175027:Experience"
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Tim Weenink (Talk | contribs) (→Applications of BBa_K175027) |
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Sequence has been shown to be cut by I-SceI in vitro. | Sequence has been shown to be cut by I-SceI in vitro. | ||
| − | === | + | ===Characterization of BBa_K175027=== |
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| + | [[Image:I6_check.png|thumb|right| Gel electrophoresis of <partinfo>BBa_K175027</partinfo> in pSB1AK3 backbone. Lane 1: undigested (supercoiled) plasmid. Lane 2: PstI digested part, expected size = 5095bp. Lane 3: PstI and I-SceI restricted part, expected band lenghts are 3204 and 1891bp. Expected lenghts correspond to the bands observed in the gel.]] | ||
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| + | This biobrick was successfully sequenced and tested for functionality. Sequencing results can be found on the part page. Functionality was assessed using commercially available I-SceI restriciton enzyme. The plasmid was purified and subjected to a digestion with BglI alone and BglI plus I-SceI. The latter would theoretically result in two fragments of 2275 and 944bp. | ||
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| + | The results in the adjecent image show that the digestion indeed results in the expected band pattern. This shows that the restriction site works as expected. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 04:13, 1 November 2009
Sequence has been shown to be cut by I-SceI in vitro.
Characterization of BBa_K175027
Gel electrophoresis of BBa_K175027 in pSB1AK3 backbone. Lane 1: undigested (supercoiled) plasmid. Lane 2: PstI digested part, expected size = 5095bp. Lane 3: PstI and I-SceI restricted part, expected band lenghts are 3204 and 1891bp. Expected lenghts correspond to the bands observed in the gel.
This biobrick was successfully sequenced and tested for functionality. Sequencing results can be found on the part page. Functionality was assessed using commercially available I-SceI restriciton enzyme. The plasmid was purified and subjected to a digestion with BglI alone and BglI plus I-SceI. The latter would theoretically result in two fragments of 2275 and 944bp.
The results in the adjecent image show that the digestion indeed results in the expected band pattern. This shows that the restriction site works as expected.
User Reviews
UNIQ31fccb71e9f9f6ba-partinfo-00000001-QINU UNIQ31fccb71e9f9f6ba-partinfo-00000002-QINU