Difference between revisions of "Part:BBa K188707"
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Revision as of 22:05, 31 October 2009
GFP regulated by tetracycline, weaker GFP expression.
This device modify from BBa_K145279, designed by iGEM08_KULeuven. It consists if a TetR generator and a GFP under the control of a TetR regulated promoter. Normally the production of GFP is switched off. It can be switched on by the addition of tetracycline(or anhydrotetracycline ). This modified device use Degenerated PCR to make different TATA box at -10 sequences. This composite is similar to BBa_K188713, but the GFP strength is slightly over than BBa_K188713.
Green fluorescence intensity was measured in Rm07 and Rm 13, two medium-low expressions in green fluorescence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1616