Difference between revisions of "Part:BBa K188634"
 
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Picture D is the tube contain great concerntration of RFP.
  
 
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Latest revision as of 21:43, 31 October 2009

RBS+mRFP+double Terminator

This device is part of device F(BBa_K188661) in our project without ccdB gene(BBa_K145151) and promoter lacI/pL(BBa_R0011). We expect that the AHL-luxR complex would bind to promoter lacI/pL(BBa_R0011) and express the the ccdB gene for E. coli suicide when project is done. And express mRFP to indicate the the project is done.

So this device only can be used to add a specific functional promoter(and other target gene), and measure the activity of the promoter by measure the expression of mRFP.

33e.jpg

Picture D is the tube contain great concerntration of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]