Difference between revisions of "Part:BBa K188151"

 
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<partinfo>BBa_K188151 short</partinfo>
 
<partinfo>BBa_K188151 short</partinfo>
  
PART OF DEVICE A OF NCTU-FORMOSA 2009 PROJECT.
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Constitutive promoter (BBa_J23106) always express the downstream genes, LacI and LuxR (BBa_C0062).  
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LacI inhibits the activity of the promoter PLac.
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If there is AHL surrounding, LuxR and AHL will form the complex and induce the expression of the promoter PcIIp22 (BBa_K145150).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:50, 31 October 2009

constitutive promoter +RBS+LacI+RBS+LuxR

Constitutive promoter (BBa_J23106) always express the downstream genes, LacI and LuxR (BBa_C0062). LacI inhibits the activity of the promoter PLac. If there is AHL surrounding, LuxR and AHL will form the complex and induce the expression of the promoter PcIIp22 (BBa_K145150).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]